Preparation and properties of the mucopeptides of cell walls of gram-negative bacteria.
نویسنده
چکیده
1. In potassium-deficient barley plants 1,4diauinobutane (putrescine), which had been previously characterized, occurs together with a Sakaguchiand ninhydrin-positive compound, whose infrared spectrum and chromatographic properties are identical with those of 1-amino-4guanidinobutane (agmatine). 2. Agmatine and putrescine occur in potassiumdeficient red-clover plants. 3. Under conditions of adequate potassium nutrition, agmatine and putrescine were found in barley and clover, and also in cabbage leaves, but the amounts were small relative to those in the potassium-deficient plants. 4. The putrescine concentration in barley seedlings was considerably increased by feeding with agmatine, a much smaller increase being induced by giving ornithine or arginine. Feeding with arginine increased the concentration of a Sakaguchipositive compound, which was chromatographically indistinguishable from agmatine. The authors thank Dr C. H. Fawcett and Dr K. Rothwell for producing the infared spectra.
منابع مشابه
Selective extraction of polymers from cell walls of gram-positive bacteria.
Brief heating of Bacillus Licheniformis cell walls at 100 degrees C in aqueous buffers of pH3.0-4.0 removes some polymers but not others from the mucopeptides. For example, relatively undegraded teichuronic acid can be extracted at 100 degrees C in 20min at pH3.0 whereas the teichoic acids are not removed. Similar specificity can be shown with walls from three other species of micro-organism.
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ورودعنوان ژورنال:
- The Biochemical journal
دوره 84 شماره
صفحات -
تاریخ انتشار 1962